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Question
Which stain is used for staining chromosomes?
Which stain is used for staining chromosomes?
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Solution
The correct option is B Acetocarmine
Acetocarmine is a DNA specific stain that stains freshly prepared unfixed super coiled chromosomes during different mitotic stages; option B is correct. Safranin is used in histology and cytology as a counterstain that colours all cell nuclei red; it is the mainly used in both Gram stains, and endospore staining; option A is incorrect. Janus Green B is a basic dye that changes colour according to the amount of oxygen present. Presence of oxygen oxidizes the indicator to blue colour while in the absence of oxygen, the reduced indicator is reduced is in pink colour; option C is incorrect. Crystal violet is used as the primary stain in Gram staining procedure. Correct options is B.
Acetocarmine is a DNA specific stain that stains freshly prepared unfixed super coiled chromosomes during different mitotic stages; option B is correct. Safranin is used in histology and cytology as a counterstain that colours all cell nuclei red; it is the mainly used in both Gram stains, and endospore staining; option A is incorrect. Janus Green B is a basic dye that changes colour according to the amount of oxygen present. Presence of oxygen oxidizes the indicator to blue colour while in the absence of oxygen, the reduced indicator is reduced is in pink colour; option C is incorrect. Crystal violet is used as the primary stain in Gram staining procedure. Correct options is B.
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