During synthesis of rat insulin through genetic engineering, the isolated DNA fragments corresponding to insulin gene were inserted in .

bacterial plasmid

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bacterial genome

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rat genome

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Solution

The correct option is A bacterial plasmid
During synthesis of rat insulin through genetic engineering, the DNA fragments corresponding to insulin gene in rat were isolated and were inserted in bacterial plasmid DNA, also containing tetracycline and penicillin gene. Subsequently, the plasmid were introduced in E. coli so that the introduced gene is expressed to form proinsulin. These products were further purified to get active insuline molecule.

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Similar questions

Given below is the image depicting the steps involved in the manufacture of insulin using bacteria through genetic engineering. Match the numbers given in the image with the steps given below.

A. Joining the two DNA segments

B. Multiplication of bacteria

C. Identification of insulin gene

D. Insertion of the new genome into bacteria

E. Cutting the gene and bacterial genome

During bacterial synthesis of insulin, the plasmid containing the insulin gene is introduced into the

Q. In genetic engineering, it is necessary to cut DNA out of bacteria and eukaryotes to insert the human insulin gene into a bacteria. Select the correct statement.

Q. Scientists have been able to splice the eukaryotic gene for the production of human insulin into the genome of bacterial cells and induce those bacteria to express the gene and produce insulin. This suggests which of the following?

Q. Genetically-engineered human insulin is obtained by inserting the gene in __________.

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