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Question

EcoRI is a restriction endonuclease. How is it named so? Explain?

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Solution

EcoRI is named so because it acts on the inner surface of DNA at specific sites and cut it into fragments, whereas exonuclease work on the ends of strands and cannot be used for the circular vector.EcoRI creates 4 nucleotide sticky ends with 5' end and overhangs at AATT.

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