Question

Which of the following statements is not correct regarding EcoRI restriction endonuclease enzyme?

• A. It is isolated from Escherichia coli RY13
• B. Its recognition sequence is 5'-GAATTC-3'
  3'-CTTAAG-5'
• C. It produces complementary blunt ends
• D. None of these

Answer 1

The correct option is C It produces complementary blunt ends

The restriction endonuclease enzyme EcoRI was isolated from bacterium Escherichia coli RY13. It recognises the base sequence GAATTC in DNA duplex and cuts its strands between G and A as shown below:5'-G-A-A-T-T-C-3'
3'-C-T-T-A-A-G-5'
It results in complementary sticky ends.