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Question

ELISA can give a false test in the beginning of an infection due to

A
Low antibody
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B
Denatured pathogen
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C
Low concentration of pathogen
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D
Antibody may not bind tightly to the titer plate
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Solution

The correct option is C Low concentration of pathogen
ELISA works on the principle of antibody-antigen interaction. In case of low concentration of pathogen it can give false test in the beginning of infection. A low concentration of pathogen will fail to produce sufficient antibodies that are required to show a positive status for a particular disease or condition thereby making it seem like an individual is negative for a disease even if in reality he/she is in fact positive for it.

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