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Question
Explain briefy.
(a) PCR
(b) Restriction enzymes and DNA
(c) Chitinase
(a) PCR
(b) Restriction enzymes and DNA
(c) Chitinase
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Solution
(a) PCR - Polymerase chain reaction technique amplify the DNA from one copy to more than one in three steps namely denaturation of target DNA (thermal cycle to separate the DNA strands), annealing of primers to the ssDNA and polymerization (extension of primer into complete DNA strand complementary to the template strand).(b) Restriction enzymes recognize specific sequences, mostly 4-6 bp, in the DNA and cut it into fragments by breaking the phosphodiester linkage between two successive nucleotides of DNA. DNA is polymer of deoxyribonucleotides and serves as genetic material(c) Chitinases are class of enzymes that can digest chitin; the major component of fungal cell wall.
(a) PCR - Polymerase chain reaction technique amplify the DNA from one copy to more than one in three steps namely denaturation of target DNA (thermal cycle to separate the DNA strands), annealing of primers to the ssDNA and polymerization (extension of primer into complete DNA strand complementary to the template strand).
(b) Restriction enzymes recognize specific sequences, mostly 4-6 bp, in the DNA and cut it into fragments by breaking the phosphodiester linkage between two successive nucleotides of DNA. DNA is polymer of deoxyribonucleotides and serves as genetic material
(c) Chitinases are class of enzymes that can digest chitin; the major component of fungal cell wall.
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