Question

Explain how suspension culture is prepared from callus?

Answer 1

For the preparation of suspension culture, callus is transferred to a liquid nutrient medium and is agitated. Cells of callus are separated due to agitation. Constant agitation at 100- 250 rpm serves the purpose of aeration, mixing and prevention of aggregation. This suspension culture is sub-cultured every week. Suspension cultures grow here more or less need to be sub-cultured rapidly than the callus culture.