Question

Commonly used auxin in callus and suspension cultures, is .............

• A. NAA
• B. IBA
• C. 2, 4-D
• D. 2, 4, 5 - Trichlorophenoxy acetic acid
• E. Abscisic acid

Answer 1

The correct option is C 2, 4-D

2, 4 - D is the synthetic auxin, which is commonly used in callus as well as suspension cultures. Both the propagation of cells, as well as the differentiation of embryos was inhabited by a continuous application of these growth regulators. For this reason, a long period on medium lacking growth regulators was necessary before the differentiation of embryos occurred again.