Question

Explain the process of synthesis of insulin by Eli Lilly Company. Name the technique used by the company.

Answer 1

In 1983, Eli Lilly an American company prepared two DNA sequences corresponding to A and B, chains of human insulin and introduced them in plasmids of E. coli to produce insulin chains. Chains A and B were produced separately, extracted and combined by creating disulfide bonds to form human insulin. Recombinant DNA technique was used for by the company.