Question

Explant is required to be disinfected before placing in culture. This is done by

• A. Autoclaving
• B. Ultra-violet rays
• C. Hypochlorite
• D. X-rays

Answer 1

Answer: (C)

Hypochlorite

Autoclaving is the technique to kill the germs by exposure to steam under high pressure and is used to sterilize the culture media and apparatus.

Ultra-violet rays are used for sterilisation of culture media is an expensive method and mostly spoils plastic apparatus.

X-rays treatment is used for deactivation of protoplast to make them serve as a donor of cytoplasmic genophores for cybrid production.

Autoclave, UV rays and X-rays are lethal to living plant tissues and thus are not used for explant sterilisation.

Hypochlorite -, sodium hypochlorite is used for sterilizing plant structures, where in the plant parts are immersed in a very dilute solution for about 15-20 minutes

So, the correct answer is option C.