Fill in the blanks from A to E for the steps involved in rDNA technology.
A: Cellulase: B: Cathode; C: Anode; D: Amplify; E: E. coli
Cell lysis is the first step involved in isolating DNA molecules from any living cell. The Lignocellulosic component of a plant cell can be digested by treating it with cellulase enzyme thus making the plant cell feasible for further isolation process.
In the process of gel electrophoresis, DNA sample along with loading dye is placed inside the well craved near the cathode side. As the current moves from cathode to anode, negatively charged DNA molecules also move towards the positively charged anode terminal.
Polymerase chain reaction is the process of amplifying a section of DNA which normally contains genes of interest.
EcoRI is a restriction endonuclease enzyme isolated from species E. coli. It cleaves DNA double helices into fragments at specific sites.