wiz-icon
MyQuestionIcon
MyQuestionIcon
1
You visited us 1 times! Enjoying our articles? Unlock Full Access!
Question

Fill in the blanks from A to E for the steps involved in rDNA technology.


A

A: Chitinase: B: Anode; C: Cathode; D: Amplify; E: E. coli

No worries! We‘ve got your back. Try BYJU‘S free classes today!
B

A: Cellulase: B: Cathode; C: Anode; D: Amplify; E: E. coli

Right on! Give the BNAT exam to get a 100% scholarship for BYJUS courses
C
A: Chitinase: B: Cathode; C: Anode; D: Separate; E: E. coli
No worries! We‘ve got your back. Try BYJU‘S free classes today!
D

A: Cellulase: B: Anode; C: Cathode; D: Isolate; E: E. coli

No worries! We‘ve got your back. Try BYJU‘S free classes today!
Open in App
Solution

The correct option is B

A: Cellulase: B: Cathode; C: Anode; D: Amplify; E: E. coli


Cell lysis is the first step involved in isolating DNA molecules from any living cell. The Lignocellulosic component of a plant cell can be digested by treating it with cellulase enzyme thus making the plant cell feasible for further isolation process.

In the process of gel electrophoresis, DNA sample along with loading dye is placed inside the well craved near the cathode side. As the current moves from cathode to anode, negatively charged DNA molecules also move towards the positively charged anode terminal.

Polymerase chain reaction is the process of amplifying a section of DNA which normally contains genes of interest.

EcoRI is a restriction endonuclease enzyme isolated from species E. coli. It cleaves DNA double helices into fragments at specific sites.


flag
Suggest Corrections
thumbs-up
0
Join BYJU'S Learning Program
similar_icon
Related Videos
thumbnail
lock
DNA FingerPrinting
BIOLOGY
Watch in App
Join BYJU'S Learning Program
CrossIcon