Question

Fill up the blanks and select the correct option.
(i) EcoRI cuts the DNA between bases ___ and ____ when the sequence _____ is present in the DNA.
(ii) Disruption of the cell membranes can be achieved by treating the bacterial cells, plant cells and fungal cells with enzymes respectively ____, ____ and ___.
(iii) Since DNA has a ____ charge, it moves towards the ____ of the electrophoretic chamber.

• A. (i) G, A, GAATTC (ii) endonuclease, cellulase, chitinase (iii) negative, anode
• B. (i) G, A, GAATTC (ii) lysozyme, cellulase, chitinase (iii) positive, cathode
• C. (i) G, A, GAATTC (ii) lysozyme, cellulase, chitinase (iii) negative/anode
• D. (i) G, A, GAAATC (ii) lysozyme, cellulase, chitinase (iii) positive, cathode

Answer 1

The correct option is C (i) G, A, GAATTC (ii) lysozyme, cellulase, chitinase (iii) negative/anode

• EcoRI cuts the DNA between bases G and A only when the sequence GAATTC is present in the DNA.
  
• Disruption of the cell membranes can be achieved by treating the bacterial cells, plant cells and fungal cells with enzymes lysozyme, cellulase and chitinases respectively.
  
• Since DNA has a negative charge , it moves towards the anode of the electrophoretic chamber.

So, the correct option is 'Option C' .