The correct option is D 1972
Recombinant DNA is formed by the introduction of a foreign DNA (containing the gene of interest) into the vector DNA. This process involves the use of restriction endonucleases and ligases.
Cohen and Boyer made the first recombinant DNA in 1972 by linking an antibiotic resistance gene with a naive plasmid from Salmonella typhimurium. This was possible due to the discovery of restriction endonuclease in the 1960s.
Hence the correct option is
d. 1972