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Electroporation

First step in...

Question

First step in Southern blot technique is

A

Digestion of DNA by restriction enzyme

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B

Production of a group of genetically identical cells

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C

Denaturation of DNA on the gel for hybridisation with specific probe

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D

Denaturation of DNA from a nucleated cell as from the scene of crime

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Solution

The correct option is A Digestion of DNA by restriction enzyme
The first step in a Southern blot technique is to prepare the DNA mixture by breaking it into smaller fragments using a restriction enzyme.

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Q. Select the correct sequences of steps in DNA fingerprinting involving southern blot hybridization using radiolabelled VNTR as probe.
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