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B
Complementary filters.
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C
Annular diaphragm.
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D
Both A and B.
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Solution
The correct option is D Both A and B. UV microscopes have commonly been used in fluorescent microscopy. In this case, the UV light that reflects the image of the sample stains to the fluorescence to create an image that can be viewed.
Fluorescence microscope differs from a microscope used for conventional light microscopy mainly in that it has a special light source and a pair of complementary filters. A fluorescence microscope uses various filters to exclude and excite specific wavelengths of light to induce fluorescence.