Following is an image that depicts PCR cycle and its important components. Identify i, ii, iii, and iv in the same.

i - Primers, ii - Deoxyribonucleotides, iii - 96℃, iv - Annealing

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i - Deoxyribonucleotides, ii - Primers, iii - 96℃, iv - Annealing

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i - Primers, ii - Deoxyribonucleotides, iii - 72℃, iv - Annealing

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i - Primers, ii - Taq polymerase, iii - 96℃, iv - Annealing

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Solution

The correct option is A i - Primers, ii - Deoxyribonucleotides, iii - 96℃, iv - Annealing
PCR or polymerase chain reaction is a technique used in molecular biology to create several copies of a certain DNA segment.

PCR occurs in three steps:

- Denaturation: The high temperature melts the hydrogen bonds and separates the two strands of double-stranded DNA. The temperature is maintained at 92-96℃.

- Annealing: The two oligonucleotide primers anneal at desired sequences in the separated strands. It occurs at 55-65℃.

- Extension: Finally, Taq DNA polymerase forms the complementary strands by adding deoxyribonucleotides at free 3' OH end of the primers. It is carried out at 72℃.

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Identify the labelled parts (I), (II) and (III) depicted in the image.

Question 23
Which one of the following depicts the correct representation of atomic radius(r) of an atom?

(a) (i) and (ii)
(b) (ii) and (iii)
(c) (iii) and (iv)
(d) (i) and (iv)

Q. From the image given below. Identify the parts (i), (ii), (iii) and (iv) respectively.

Identify the components depicted in the given image.

Q. Arrange the steps of diagnosing AIDS using the PCR method in the right order:
I - cDNA, engineered primers, and nucleotides along with buffer are incubated in a thermal cycler
II - Primers specific to HIV genes are engineered
III - RNA is extracted from the patient’s plasma
IV - The PCR product is run on a gel to separate and identify the PCR amplified viral genes
V - Reverse transcriptase is used to convert the RNA to cDNA

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