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Question

Following is an image that depicts PCR cycle and its important components. Identify i, ii, iii, and iv in the same.


A
i - Primers, ii - Deoxyribonucleotides, iii - 96℃, iv - Annealing
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B
i - Deoxyribonucleotides, ii - Primers, iii - 96℃, iv - Annealing
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C
i - Primers, ii - Deoxyribonucleotides, iii - 72℃, iv - Annealing
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D
i - Primers, ii - Taq polymerase, iii - 96℃, iv - Annealing
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Solution

The correct option is A i - Primers, ii - Deoxyribonucleotides, iii - 96℃, iv - Annealing
PCR or polymerase chain reaction is a technique used in molecular biology to create several copies of a certain DNA segment.

PCR occurs in three steps:

- Denaturation: The high temperature melts the hydrogen bonds and separates the two strands of double-stranded DNA. The temperature is maintained at 92-96℃.

- Annealing: The two oligonucleotide primers anneal at desired sequences in the separated strands. It occurs at 55-65℃.

- Extension: Finally, Taq DNA polymerase forms the complementary strands by adding deoxyribonucleotides at free 3' OH end of the primers. It is carried out at 72℃.


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