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Ligation of DNA

For ligation,...

Question

For ligation, the vector and the targetted gene should be cut by restriction enzyme.

A

same

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B

any

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C

random

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D

different

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Solution

The correct option is A same
The vector and gene should be cut by the same restriction enzyme, so that the vector and gene can join together. The restriction enzyme generates complementary ends which are easy to join.

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Q. The vector plasmid and the gene of interest that have been cut with the same restriction enzyme creating sticky ends are joined by .

Q. Assertion :Recognition site should be preferably single and responsive to commonly used restriction enzyme. Reason: In pBR322 alien DNA is ligated generally in the area of Bam HI site of tetracyline resistance gene.

Q. Assertion :Recognition site should be preferably single and responsive to commonly used restriction enzymes. Reason: In

p B R 322

D N A

is ligated generally in the area of

B a m - H I

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Q. Arrange the given steps involved in recombinant DNA technology in the correct order.

A. Amplification of desired DNA sequence (gene of interest) with PCR modification
B. Ligation of gene of interest with the vector
C. Removal of RNA and proteins to purify DNA
D. Insertion of recombinant DNA into the host
E. Treatment of cell with cell wall digesting enzyme followed by disruption of the cell membrane
F. Digestion of DNA of interest and vector with suitable restriction enzyme

Q. Write the types of restriction enzyme. "Synthesis of recominant DNA molecule is possible only when the vectors and source DNA is cut by the same restriction enzyme" explain reason.

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Ligation of DNA

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