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Question
Fragments of DNA formed after treatment with endonucleases are separated by the technique
Fragments of DNA formed after treatment with endonucleases are separated by the technique
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Solution
The correct option is D Electrophoresis
Digestion of DNA is done using restriction endonucleases, to cut DNA at specific sites and make required fragments.
Polymerase chain reaction (PCR) is a technique used for the in vitro amplification of the DNA fragments or gene of interest to create multiple copies.
Southern blotting is an analytical tool used for the specific identification of desired DNA from thousands of DNA molecules.
Colony hybridization is a technique used to select bacterial colonies with the desired gene. In this method, a colony is picked followed by DNA isolation. The specific gene is then detected by using radioactive or fluorescent labelled probes.
Electrophoresis is the movement of charged particles in an electric field. It is used for the separation of DNA fragments formed after treatment with endonucleases, based on their size. In this method, the DNA fragments being negatively charged, moves towards the positive electrode during gel electrophoresis.
Hence the correct option is
d. Electrophoresis
Digestion of DNA is done using restriction endonucleases, to cut DNA at specific sites and make required fragments.
Polymerase chain reaction (PCR) is a technique used for the in vitro amplification of the DNA fragments or gene of interest to create multiple copies.
Southern blotting is an analytical tool used for the specific identification of desired DNA from thousands of DNA molecules.
Colony hybridization is a technique used to select bacterial colonies with the desired gene. In this method, a colony is picked followed by DNA isolation. The specific gene is then detected by using radioactive or fluorescent labelled probes.
Electrophoresis is the movement of charged particles in an electric field. It is used for the separation of DNA fragments formed after treatment with endonucleases, based on their size. In this method, the DNA fragments being negatively charged, moves towards the positive electrode during gel electrophoresis.
Hence the correct option is
d. Electrophoresis
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