Question

Fragments of DNA formed after treatment with endonucleases are separated by the technique

• A. Polymerase chain reaction
• B. Southern blotting
• C. Colony hybridisation
• D. Electrophoresis

Answer 1

The correct option is D Electrophoresis

Gel electrophoresis uses a positively charged grid to attract the negatively charged DNA fragments, thereby separating them by size, because the smaller ones will migrate the most. Radioactive or fluorescent probes are added, which attract and bind with the desired DNA to produce visible bands.

So, option D is the correct option.