Question

Fungi can be stained by

• A. Cotton blue
• B. Safranine
• C. Glycerine
• D. Lactophenol

Answer 1

Answer: (A), (D)

The correct options are
A Cotton blue
D Lactophenol

• Stains such as Cotton blue and Lactophenol are used to stain fungi cells to study them under microscope.
• These stain color the cell wall and the nucleus of the fungus cell blue so that they stand out when observed under the microscope.
• The lactophenol is used along with cotton blue dye. The phenol is fungicidal which kills the organism and works as a cleansing agent where as lactic acid here works as a preservative to preserve the sample. The cotton blue is used to stain the colorless cytoplasm of the fungus so when observed under the microscope the structure of the fungus is observed with the light blue back ground.
• Therefore the option A or D is the correct answer.