The correct option is B Due to change in sequence of nitrogenous base
Reproduction is the production of offspring by sexual or asexual means; it does not cause mutation; option A is incorrect. The linkage is the tendency of closely placed genes on a chromosome to stay together during inheritance; it produces more parental combination and less/no new combinations of the gene, option B is incorrect. Mutation in single nucleotide base of a DNA segment is called as point/gene mutation. It can introduce a premature stop codon, or a nonsense codon, by addition or deletion of the base, in the transcribed mRNA (nonsense mutation) or changes a codon specifying a different amino acid by substitution of one base (missense mutation); option C is correct. The nucleotide sequence of each gene determines the protein product; not the sequence of genes on chromosomes. Thus, any changes in gene sequence do not cause mutation; option D is incorrect.