Gram-positive bacteria | Gram negative bacteria |
They remain coloured blue or purple with Gram stain even after washing with absolute alcohol or acetone. | The bacteria do not retain the stain when washed with absolute alcohol. |
The wall is single layered. Outer membrane is absent. | The wall is two layered. Outer membrane is present. |
The thickness of the wall is 20-80 nm. | It is 8-12 nm. |
The lipid content of the wall is quite low. | The lipid content of the wall is 20-30%. |
The wall is straight. | The wall is wavy and comes in contact with plasmalemma at a few places. |
Murein or mucopeptide is 70-80%. | It is 10-20%. |
Porins are absent. | Porins or hydrophilic channels occur in outer membrane of cell wall. |
Cell wall contains teichoic acids. | Teichoic acids are absent. |