Question

Give the full form of ELISA. Which disease can be detected using it? Discuss the principle under lying the test.

Answer 1

Full form of ELISA:
ELISA stands for Enzyme linked Immuno-sorbent Assay.



Diseases identified by ELISA:
ELISA is a test for the diagnosis of HIV/AIDS or checking the levels of hormones like thyroxine in the body.



Principle of ELISA:
Antigen-antibody interaction and the specific identification of this complex is the main principle behind ELISA technique.The antibody formed in the body in response to the infection is detected by its interaction with the specific antigen. Later the antigen- antibody complex is identified using an anti antibody, substrate and enzyme..