Question

Given figures represent the steps involved in polymerase chain reaction (PCR). Identify the steps A, B, C and D, and select the correct option.

	A	B	C	D
a	Denaturation at 94-96 degree C	Extension through Taq polymerase at 72 degree C	Repetition of denaturation and annealing	Annealing at 72 degree C
b	Denaturation at 94-96 degree C	Annealing at 40-60 degree C	Extension through Taq polymerase at 72 degree C	Repetition of denaturation and annealing
c	Denaturation at 40-60 degree C	Annealing at 72 degree C	Extension through Taq polymerase at 94-96 degree C	Repetition of denaturation and annealing
d	Extension through Taq polymerase at 72 degree C	Denaturation at 40 degree C	Annealing at 72 degree C	Repetition of denaturation and annealing

• A. a
• B. b
• C. c
• D. d

Answer 1

Answer: (B)

b

Polymerase chain reaction involves the steps:

• Denaturation
• Annealing
• Extension
• Amplification

So, the correct option is 'Option B ' .