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How are YACs constructed?
How are YACs constructed?
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Solution
YACs
- Yeast artificial chromosome (YAC) vectors have one centromere (CEN), two telomeric sequences (TEL), one autonomously replicating sequence (ARS), and the genes for selectable markers in yeast.
- YACs are constructed by joining these elements which are needed for the segregation and replication of the yeast chromosomes.
- Yeasts containing YACs grown on selective media that lack particular proteins.
- However, the selectable markers do not offer antibiotic resistance like Escherichia coli plasmids.
YACs
- Yeast artificial chromosome (YAC) vectors have one centromere (CEN), two telomeric sequences (TEL), one autonomously replicating sequence (ARS), and the genes for selectable markers in yeast.
- YACs are constructed by joining these elements which are needed for the segregation and replication of the yeast chromosomes.
- Yeasts containing YACs grown on selective media that lack particular proteins.
- However, the selectable markers do not offer antibiotic resistance like Escherichia coli plasmids.
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