Question

How can we separate the RNA from the sample? Is it easy and less expansive?

Answer 1

1. Amplification can be done with RNA templates following the same procedure as with DNA templates, simply through the addition of reverse transcriptase.
2. The target sequence is amplified at a constant temperature of $60-{65}^{\circ }C$
3. The total cost can be reduced, as LAMP dosenot require special reagents or sophisticated equipments. Cost is low compare to PCR.
4. The amplification efficiency is extremely high.
5. Single tube is enough. But We should maintain the isothermal condition.
6. By use of dye we detect the product after electrophoresis
7. Crude DNA from sample is enough to run LAMP
8. You can use tubidimeter to identify the product

Drawbacks
Less sensitive than PCR
Less versatile than PCR
Not useful for cloning
Less freedom to choose the target site than with PCR