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How does dot blot start?
How does dot blot start?
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Solution
Dot blot
- Dot blot, also called slot blot is a technique used in molecular biology to detect proteins.
- It starts with spotting the RNA or DNA samples onto the nylon membrane.
- This nylon membrane is developed with a radioactive probe and then exposed to photographic films.
- Hybridization occurs between the single-stranded DNA or RNA strands with the single-stranded probes.
- The samples that contain RNA or DNA complementary to the given probe will give a black spot on the film.
- Various concentrations of the sample can be dotted in a side-by-side pattern on the membrane.
- This technique can also be used for protein detection using primary and secondary antibodies as shown below:
Dot blot
- Dot blot, also called slot blot is a technique used in molecular biology to detect proteins.
- It starts with spotting the RNA or DNA samples onto the nylon membrane.
- This nylon membrane is developed with a radioactive probe and then exposed to photographic films.
- Hybridization occurs between the single-stranded DNA or RNA strands with the single-stranded probes.
- The samples that contain RNA or DNA complementary to the given probe will give a black spot on the film.
- Various concentrations of the sample can be dotted in a side-by-side pattern on the membrane.
- This technique can also be used for protein detection using primary and secondary antibodies as shown below:
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