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Question

An analysis of chromosomal DNA using the Southern blot by hybridization technique does not use

A
PCR
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B
Electrophoresis
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C
Autoradiography
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D
Blotting
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Solution

The correct option is A PCR

Conclusion

Polymerase chain reaction or PCR is a reaction carried out in vitro to amplify a specific DNA sequence or gene of interest and create its multiple copies.
  • PCR is used in DNA fingerprinting only when the DNA isolated from the biological sample is in very small amounts.
  • Thus, it is not mandatory to perform PCR in DNA fingerprinting.

Final answer: (a) PCR

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