Question

How foreign DNA is inserted into a plasmid vector?

Answer 1

DNA inserted into a plasmid vector process:

1. Molecular cloning is the technique of selectively replicating any piece of DNA.
2. This can be accomplished through the use of recombinant DNA or the polymerase chain reaction.
3. The method of combining DNA from two distinct species is known as recombinant DNA technology.
4. The recombinant DNA is subsequently given to a host in order to create novel combinations.
5. This procedure is completed in three steps: the DNA is cut, joined, and finally inserted using vectors
6. Conjugative plasmids make up the majority of resistance plasmids. E.coli is employed to clone vectors in this scenario.
7. As a result of the deletion of the original genes, the plasmids no longer release antibacterial toxins.
8. The antibiotic resistance gene is then inserted, which aids in the identification of bacteria with plasmid changes.