Question

How has the use of Agrobacterium as vectors helped in controlling Meloidogyne incognita infestation in tobacco plants? Explain in correct sequence. [3]

Answer 1

[3]
(i) Using Agrobarterium vector, nematode specific genes are introduced into the host plant.
(ii) Both sense and antisense strands of m-RNA are produced.
(iii) Double stranded RNA (ds RNA) is formed.
(iv) dsRNA initiates RNA interference.
(v) It prevents translation of mRNA resulting in silencing of mRNA of parasite or nematode.
(vi) This causes the parasite Meloidogyne incognita to die.