Question

How many copies of the DNA sample are produced in PCR technique after 6-cycle?

• A. $4$
• B. $32$
• C. $64$
• D. $16$

Answer 1

The correct option is C $64$

Polymerase chain reaction (PCR) is a technique that exponentially amplifies a single copy of a specific DNA segment. It generates thousands of copies of that specific DNA segment. After completion of one cycle, 2 copies are produced from a single DNA segment. After completion of the second cycle, 2${}^2$ = 4 copies are produced. Similarly, after n${}^{th}$ cycle, 2${}^2$ copies are produced, where n is the number of cycles. Hence, after completion of 6 cycle, 2${}^6$ = 64 copies will be produced.

Thus, the correct answer is '64.'