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Question
How many sticky ends will be produced if a plasmid vector is digested at a single site by EcoR I?
How many sticky ends will be produced if a plasmid vector is digested at a single site by EcoR I?
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Solution
The correct option is B 2
Plasmids are autonomously replicating, extrachromosomal, small, circular double stranded DNA found in many bacteria. As it can replicate on its own it is used as a vector for transferring the desired DNA into the suitable host.
Restriction endonucleases are the enzymes that cleave the phosphodiester bonds present between consecutive nucleotides within specific DNA sequences known as recognition sites.
EcoR I is a restriction endonuclease. It has been isolated from the bacteria Escherichia coli RY13. It recognises the base sequence 5’ GAATTC 3’ in the double stranded DNA and makes the cuts between G and A. As the cut is made slightly away from the centre, it results in the formation of DNA fragments with single stranded overhanging free ends called sticky ends.
The figure below depicts that it makes the cuts between G and A in both the strands in the plasmid DNA. This results in the formation of a DNA fragment with two sticky ends.
Hence, two sticky ends are produced if a plasmid vector is digested at a single site by EcoR I.
Plasmids are autonomously replicating, extrachromosomal, small, circular double stranded DNA found in many bacteria. As it can replicate on its own it is used as a vector for transferring the desired DNA into the suitable host.
Restriction endonucleases are the enzymes that cleave the phosphodiester bonds present between consecutive nucleotides within specific DNA sequences known as recognition sites.
EcoR I is a restriction endonuclease. It has been isolated from the bacteria Escherichia coli RY13. It recognises the base sequence 5’ GAATTC 3’ in the double stranded DNA and makes the cuts between G and A. As the cut is made slightly away from the centre, it results in the formation of DNA fragments with single stranded overhanging free ends called sticky ends.
The figure below depicts that it makes the cuts between G and A in both the strands in the plasmid DNA. This results in the formation of a DNA fragment with two sticky ends.
Hence, two sticky ends are produced if a plasmid vector is digested at a single site by EcoR I.
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