Question

If a plasmid vector is digested with EcoRI at a single site, then

• A. One sticky end will be produced
• B. Two sticky ends will be produced
• C. Four sticky ends will be produced
• D. Six sticky ends will be produced

Answer 1

The correct option is B Two sticky ends will be produced

A vector is a vehicle, usually an artificial DNA molecule, that carries a foreign genetic material into the cell. In this cell, it gets replicated. Restriction endonuclease enzymes create sticky ends that are then useful in producing a recombinant DNA molecule. There are 2 types of ends, Sticky ends and blunt ends. They cut the DNA strands 4 base base pairs from each other and create 5' overhang in one direction and complementary 5' overhang in another. When a 5' overhang remains it means that stranded base has ended in 5' phosphate region and similar is for 3' overhang.

So the correct answer "Two sticky ends will be produced".