The correct option is B 1-I, 2-II, 3-III
1. Segment representing the energy of activation. The activation energy is the energy required to start a reaction.
2. Segment representing the amount of free energy released by the reaction.
3. In order for the reaction to take place, some or all of the chemical bonds in the reactants must be broken so that new bonds, those of the products, can be formed. During the state where substrate is bound to the enzyme active site, a new structure of the substrate called transition state structure is formed. It is unstable.