Identify the correct sequence of steps involved in the process of gel electrophoresis:
Identify the correct sequence of steps involved in the process of gel electrophoresis:
The correct option is C 8, 6, 7, 5, 3, 4, 2, 1
Agarose gel electrophoresis is a laboratory technique commonly used to separate charged particles (like DNA) of varying sizes in an agarose matrix under the influence of an electric field. The technique involves the following steps:-
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Agarose powder is dissolved in TAE or TBE buffer. Ethidium bromide is also dissolved in it. It is casted into the tray.
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Combs are inserted into the gel. It is then cooled for gel solidification.
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After solidification, the combs are removed to obtain wells.
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DNA samples are pipetted into the wells.
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Buffer is added to the electrophoresis chamber.
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Power is turned on to allow current conduction.
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Once migration is done, the power supply is turned off.
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The gel is procured out and viewed under UV light.
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The part of the gel containing the gene of interest is identified and excised.
-
Elution of DNA is done to get the gene of interest.
Agarose gel electrophoresis is a laboratory technique commonly used to separate charged particles (like DNA) of varying sizes in an agarose matrix under the influence of an electric field. The technique involves the following steps:-
-
Agarose powder is dissolved in TAE or TBE buffer. Ethidium bromide is also dissolved in it. It is casted into the tray.
-
Combs are inserted into the gel. It is then cooled for gel solidification.
-
After solidification, the combs are removed to obtain wells.
-
DNA samples are pipetted into the wells.
-
Buffer is added to the electrophoresis chamber.
-
Power is turned on to allow current conduction.
-
Once migration is done, the power supply is turned off.
-
The gel is procured out and viewed under UV light.
-
The part of the gel containing the gene of interest is identified and excised.
-
Elution of DNA is done to get the gene of interest.
