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Question

Identify the enzymes catalysing reactions (A) and (B)

(A)α-ketoglutarate + NH4+ + NADPH Glutamate + H2O + NADP+

(B)RCH(COO)NH2+R1(CO)COO R(CO)COO+R1CH(COO)NH2


A

A - Glutamate dehydrogenase, B - Transaminase

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B

A - Transaminase, B - Glutamate dehydrogenase

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C

A - Nitrogenase, B - Transaminase

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D

A - Glutamate aminase, B - Dehydrogenase

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Solution

The correct option is A

A - Glutamate dehydrogenase, B - Transaminase


In reductive amination, ammonia reacts with α-ketoglutaric acid and forms glutamic acid in the presence of glutamate dehydrogenase. Transamination involves the transfer of amino group from an amino acid to keto group of a keto acid in the presence of transaminase.


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