If proteases are not used during DNA extraction, what would be the end result?

DNA would be cut into small pieces by nucleases

No worries! We‘ve got your back. Try BYJU‘S free classes today!

Helicase can unwind the DNA once the cell is ruptured

No worries! We‘ve got your back. Try BYJU‘S free classes today!

DNA can bind to certain proteins once it is out of nuclear membrane and hence they need to be destroyed

No worries! We‘ve got your back. Try BYJU‘S free classes today!

DNA is bound with histone proteins that needs to be digested

Right on! Give the BNAT exam to get a 100% scholarship for BYJUS courses

Open in App

Solution

The correct option is D DNA is bound with histone proteins that needs to be digested
Within the nucleus, the DNA is found in association with histone proteins. DNA is twisted and coiled several times around these proteins giving rise to chromatin structure. Without removing histones, we cannot isolate DNA.Therefore, we use proteases during DNA extraction.

Suggest Corrections

Similar questions

Question 31
Mitosis results in producing two cells which are similar to each other. What would be the consequence if each of the following irregularities occur during mitosis?
Nuclear membrane fails to disintegrate

Mitosis results in producing two cells which are similar to each other. What would be the consequence if each of the following irregularities occur during mitosis?
Duplication of DNA does not occur

Mitosis results in producing two cells which are similar to each other. What would be the consequence if each of the following irregularities occur during mitosis?
Centromeres do not divide

Mitosis results in producing two cells which are similar to each other. What would be the consequence if each of the following irregularities occur during mitosis?
Cytokinesis does not occur.

Q. If the “annealing” step is missed during PCR, what would be its effect on the entire process?
I. DNA strands will not separate
II. Primers will not attach
III. DNA Polymerase would not work
IV. DNA will not extend

Q. Restriction enzymes that are used in the construction of recombinant DNA are endonucleases which cut the DNA at 'specific-recognition sequence'. What would be the disadvantage if they do not cut the DNA at specific-recognition sequence?

Q. Assertion :To extract RNA from tomato cells it must first be treated with cellulase, protease and then deoxyribonuclease. Reason: Deoxyribonuclease will digest DNA while RNA will be intact.

Q. During extraction of metals, electrolytic refining is used to obtain pure metals. What is the electrolyte used?

Join BYJU'S Learning Program