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Question

If purified sample of DNA is kept in water, then the solution becomes _______.

A
Acidic
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B
Basic
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C
Neutral
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D
No effect
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Solution

The correct option is B Acidic
  • The phosphate group in the DNA molecule is present as a phosphoric acid molecule that can donate two protons and is negatively charged, thereby imparting acidic nature to DNA.
  • If one kept purified DNA in water (pH between 6.5 ton8.5), the overall pH of the solution becomes acidic.
  • But the chance of acid hydrolysis of DNA is high in water which can be prevented by storing DNA into the TE (Tris + EDTA) buffer.
So the correct answer is option A.

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