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Question

If u have a dense laboratory culture of bacteria in a petri dish what is the best measure to report its density?

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Solution

  1. Standard plate count/Colony forming units (CFUs): Manual Colony count upto 30, afterwards will be mentioned as Too numerous to count (TNTC)
  2. Serial dilution with standard suspension(made up of McFarland Turbidity Standards)
  3. Direct microscopic count
  4. Turbidimetric Measurement: Measurement of cell concentration in suspension by optical density

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