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Question

In animal tissue culture which of the following radioactive nucleotide is used to measure RNA synthesis?

A
Adenine
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B
Cytosine
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C
Guanine
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D
Thymine
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E
Uracil
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Solution

The correct option is E Uracil
RNA differs from DNA in having uracil base in place of thymine. So, RNA has adenine and uracil as purines and cytosine and guanine as pyrimidines. The purine bases of DNA are adenine and thymine while the pyrimidine bases are cytosine and guanine. Radiolabelled uracil bases can be used for measurement of RNA synthesis as uracil will be incorporated in RNAs only. Thus, the correct answer is option E.

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