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Nucleolus

In density gr...

Question

In density gradient centrifugation of human DNA, two bands are obtained. The lighter band represents

A

Bulk DNA

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B

Genomic DNA

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C

Satellite DNA

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D

Constitutive DNA

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Solution

The correct option is C Satellite DNA
If we perform density gradient centrifugation with a Human DNA sample we will obtain two different bands. The bottom layer(heavier band) will consists of the denser DNA sequence and top layers (lighter band) will consists of the part of DNA which is less dense.
The bands are different because the repetitive sequences have a base composition distinct from the genome average, which allows it to form a separate fraction by virtue of its distinct buoyant density. Because it forms a separate band from the non-repetitive sequences of DNA, this lighter band of DNA is known as "satellite DNA".

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