Question

Study the given figure carefully and select the incorrect statements regarding this.(i) It represents a typical agarose gel electrophoresis in which lane 1 contains undigested DNA(ii) Smallest DNA bands are formed at A and largest DNA bands are formed at B(iii) The separated DNA fragments can be visualized after staining in the visible light(iv) The separated DNA bands are cut out from the agarose gel and extracted from the gel piece. This step is known as elution.

• A. (i) and (ii)
• B. (ii) and (iii)
• C. (ii) and (iv)
• D. (i) and (iv)

Answer 1

The correct option is B (ii) and (iii)

The DNA fragments separate according to their size through the agarose gel, with smaller fragments moving farther away as compared to larger ones. The DNA fragments can be visualised by staining them with ethidium bromide followed by exposure to UV radiations.