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Nucleolus

In density gr...

Question

In density gradient centrifugation of human DNA, two bands are obtained. The lighter band represents

A

Bulk DNA

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B

Genomic DNA

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C

Satellite DNA

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D

Constitutive DNA

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Solution

The correct option is C Satellite DNA
If we perform density gradient centrifugation with a Human DNA sample we will obtain two different bands. The bottom layer(heavier band) will consists of the denser DNA sequence and top layers (lighter band) will consists of the part of DNA which is less dense.
The bands are different because the repetitive sequences have a base composition distinct from the genome average, which allows it to form a separate fraction by virtue of its distinct buoyant density. Because it forms a separate band from the non-repetitive sequences of DNA, this lighter band of DNA is known as "satellite DNA".

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Q. In density gradient centrifugation, satellite DNA forms ___________.

Q. Meselson and Stahl grew bacteria in the presence of a heavy isotope of nitrogen N

^{14}

. The DNA was then transferred to a growth medium containing nucleotides with a lighter isotope of nitrogen N

^{12}

which then proceeded to replicate.
The DNA was centrifuged to separate it into weight forming bands. After one round of DNA replication, there was one band in the middle of the centrifuge tube. After two rounds of DNA replication in the lighter nitrogen, two separate bands of DNA could be seen. This demonstrated that replication is semi-conservative.
Why do these results support that idea?

Is the whole genome used for dna fingerprinting or only vntr''s after density gradient centrifugation?

Q. In the eukaryotic genome
(A) Repetitive DNA is represented as a different peak in density gradient centrifugation
(B) The classification of microsatellite and minisatellite is based on the base composition, length of the segment, and number of repetitive units
(C) Coding DNA forms a large portion of the human genome
(D) Repetitive DNA shows high degree of polymorphism

Choose the correct option

Q. Study the given figure carefully and select the incorrect statements regarding this.(i) It represents a typical agarose gel electrophoresis in which lane 1 contains undigested DNA(ii) Smallest DNA bands are formed at A and largest DNA bands are formed at B(iii) The separated DNA fragments can be visualized after staining in the visible light(iv) The separated DNA bands are cut out from the agarose gel and extracted from the gel piece. This step is known as elution.

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