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Question

In gel electrophoresis, separated DNA fragments can be visualized with the help of

A
acetocarmine in UV radiation
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B
ethidium bromide in infrared radiation
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C
acetocarmine in bright blue light
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D
ethidium bromide in UV radiation
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Solution

The correct option is D ethidium bromide in UV radiation
The separated DNA fragments can be visualised only after staining the DNA with a compound known as ethidium bromide followed by exposure to UV radiation as we cannot see pure DNA fragments in the visible light and without staining. We generally observe bright orange coloured bands of DNA in a ethidium bromide stained gel when exposed to UV light.

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