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Griffith's Experiment

In Griffith’s...

Question

In Griffith’s transformation experiment, the conversion of R strain to S strain was due to:

A

Transfer of transforming principle from live R strain to heat-killed S strain bacteria

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B

Uptake of naked DNA from medium by heat-killed S strains

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C

Transfer of transforming principle from heat-killed S strains to live R strains

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D

Uptake of transforming principle from medium by heat-killed S strains to become live S strains.

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Solution

The correct option is C Transfer of transforming principle from heat-killed S strains to live R strains
The transforming principle, later identified as DNA, passed on from the heat-killed S strains enabled the R strains to synthesise a smooth polysaccharide capsule and become virulent.

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Q. Assertion :In Griffiths experiment, a mixture of heat-killed virulent bacteria R and live non-virulent bacteria 5, lead to the death of mice. Reason: Transforming principle got transferred from heat-killed R strain to S strain and made it virulent.

Q. To describe transforming principle, Frederick Griffith performed series of experiments by selecting two strains of Streptococcus pneumoniae namely S-strain (virulent) and R-strain (non-virulent).
Which of the following steps does not describe this experiment?
[0.7 mark]

Q. In Griffith experiments, what happened when heat-killed S strains pneumococci were injected into a mouse along with live R strains pneumococci ?

Q. Avery, MacLeod and Mc. Carty used the S(virulent) and R(avirulent) strains of D.pneumococci. They isolated and purified proteins, DNA, RNA, carbohydrates and lipids from the S strain. They treated the living avirulent R strain with each of these chemicals and Identify the transforming principle (DNA).
R-type + Protein S-type

\to

A-type
R-type + Carbohydrate S-type

\to

B-type
R-type + DNA of S-type + DNAase

\to

C-type
R-type + DNA of S-type

\to

D-type
Identify the type of bacteria(A to D)

Q. (a) In an experiment Griffith introduced heat-killed S-strain bacteria Streptococcus into a mouse along with live R-strain bacteria. The mouse died but the scientist recovered living S-strain bacteria from the dead mouse. Explain the conclusion Griffith arrived at after the experiment.
(b) Explain MacLeod, McCarty and Averys work that followed Griffith's experiment. State the conclusion they arrived at.

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