In pBR322, tetracycline resistance gene ( $t e t^{R}$ ) has recognition site for which of the following restriction endonuclease?

HindIII

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BamHl

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EcoRI

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Pstl

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Solution

The correct option is C BamHl
In plasmid vector pBR322, two unique restriction sites PstI arid PvuI are located within the $a m p^{R}$ gene and BamHl, SaII, etc., are located within the $t e t^{R}$ gene. The presence of restriction sites within the marker genes $t e t^{R}$ and $a m p^{R}$ permits an easy selection for cells transformed with the I recombinant pBR322. When restriction enzyme BamHl or SaII is used, the DNA insert is placed within the gene $t e t^{R}$ making it nonfunctional.
So, the correct answer is 'BamHl'.

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