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Question
In PCR, short primers are added to single stranded DNA molecules in a test tube and the appropriate enzymes are included to make a copy of the DNA. Which of the following primers is needed for copying the single stranded DNA sequence 5′TACGGTAGGTC3′ ?
In PCR, short primers are added to single stranded DNA molecules in a test tube and the appropriate enzymes are included to make a copy of the DNA. Which of the following primers is needed for copying the single stranded DNA sequence 5′TACGGTAGGTC3′ ?
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Solution
The correct option is A 5′ATGCC3′
A primer is a short single strand of RNA or DNA (generally about 18-22 bases) that serves as a starting point for DNA synthesis. It is required for DNA replication because the enzymes that catalyze this process, DNA polymerases, can only add new nucleotides to an existing strand of DNA. The polymerase starts replication at the 3′-end of the primer, and copies the opposite strand.
For copying the single stranded DNA sequence 5′TACGGTAGGTC3′, complementary sequence possessing primer is needed which is 5′GACCT3′.
So the correct option is '5′GACCT3′'.
A primer is a short single strand of RNA or DNA (generally about 18-22 bases) that serves as a starting point for DNA synthesis. It is required for DNA replication because the enzymes that catalyze this process, DNA polymerases, can only add new nucleotides to an existing strand of DNA. The polymerase starts replication at the 3′-end of the primer, and copies the opposite strand.
For copying the single stranded DNA sequence 5′TACGGTAGGTC3′, complementary sequence possessing primer is needed which is 5′GACCT3′.
So the correct option is '5′GACCT3′'.
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