In prokaryotes, the process of replication is catalysed by the following enzymes. Identify which of the enzymes is best coordinated with the role?

Helicase

\to

Joins the ends of DNA segments

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DNA polymerase I

\to

Synthesis of DNA

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DNA polymerase II

\to

Erases primer and fills gaps

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Primase

\to

Synthesis RNA primers

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Solution

The correct option is D Primase $\to$ Synthesis RNA primers
DNA primase is an enzyme involved in the replication of DNA. DNA primase is a type of RNA polymerase which synthesizes RNA primer. This RNA primer is then extended by DNA polymerase to replicate ssDNA. Primase is an important enzyme of primosome complex.
Thus, the correct answer is option D.

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Match the following enzymes of DNA replication with their respective functions:

p. DNA polymerase i. Helps unzip the DNA double helix

q. DNA ligase ii. Seals nicks between Okazaki fragments

r. DNA helicase iii. Lays RNA primers to which polymerase can add nucleotides

s. Primase iv. Adds nucleotides to the free 3’ end of RNA primers

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Q. Which of the following contains the key tools for recombinant DNA technology?
(i) Restriction endonucleases, ligases, vectors(ii) Ligases, host organism, polymerase enzymes(iii) Vectors, Taq polymerase, primers(iv) Restriction exonucleases, ligases, primers, bioreactors

Q. How many of the following statements are correct ?

A. DNA dependent DNA polymerase requires DNA primer for initiating replication but DNA dependent RNA polymerase does not require any primer for transcription.

B. DNA dependent DNA polymerase requires RNA primer for initiating replication but DNA dependent RNA polymerase does not require any primer for transcription.

C. During replication, unwinding of DNA double helix forms a fork-like structure called replication fork.

D. During transcription, unwinding of the DNA double helix by DNA dependent RNA polymerase forms a transcription bubble.

E. During transcription, unwinding of DNA double helix by DNA dependent RNA polymerase forms a transcription fork.

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