Question

In recombinant DNA technology, a plasmid vector is cleaved by ________________.

• A. Modified DNA ligase
• B. A heated alkaline solution
• C. The same enzyme that cleaves the donor DNA
• D. The different enzyme than that cleaves the donor DNA

Answer 1

The correct option is C The same enzyme that cleaves the donor DNA

Plasmid vector is cleaved by the same restriction enzyme that cleaves donor DNA leading to the creation of sticky ends.

So, the correct option is C.