During bacterial synthesis of insulin, the plasmid containing the insulin gene is introduced into the
The Human Genome contains the gene that codes for insulin. This gene can be isolated and used as a gene of interest and inserted into a plasmid. The plasmid DNA is extracted from the bacteria and cut using a restriction enzyme. Now the plasmid and the gene coding for insulin are allowed to ligate using the enzyme ligase. Now this modified plasmid that contains the gene coding for insulin is introduced to the bacterial cell. When this bacteria is provided with a favourable medium they produce insulin which can be used by humans.